Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 14)

appendix as a guide for diluting the protein
standard. (The dilutions in the tables are
enough for performing triplicate measure-
ments of the standards.) For the diluent, use
the same buffer as in the samples (refer to
Troubleshooting section for more informa-
tion). Protein solutions are normally assayed
in duplicate or triplicate. For convenience,
the BSA or gamma-globulin standard sets
can be used, but blank samples (0 µg/ml)
should be made using water and dye
reagent.
4. Pipet each standard and unknown sample
solution into separate clean test tubes or
microplate wells (the 1 ml assay may be
performed in disposable cuvettes). Add the
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