Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 35)

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Microplate Microassay Dilutions for BSA or
Gamma-Globulin Standard Sets
Standard Source of Diluent Final
Tube # Volume (µl) Standard Volume (µl) [Protein] (µg/ml)
1 10 2 mg/ml 790 25
2 5 2 mg/ml 495 20
3 5 1.5 mg/ml 495 15
4 5 1 mg/ml 495 10
5 5 0.5 mg/ml 495 5
6 5 0.25 mg/ml 495 2.5
7 5 0.125 mg/ml 495 1.25
8 (blank) – – 500 0
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