Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 9)

Table 1. Reagents compatible with the
Quick Start Bradford protein assay when
using the standard procedure.*
7
Acetone, 10%
Acetonitrile, 10%
Ammonium sulfate, 1 M
Ampholytes, 3–10, 0.5%
ASB-14, 0.025%
Ascorbic acid, 50 mM
Bis-Tris, pH 6.5, 0.2 M
b-mercaptoethanol, 1 M
Calcium chloride, 40 mM
CHAPS, 10%
CHAPSO, 10%
Deoxycholic acid, 0.2%
DMSO, 5%
Dithioerythritol (DTE), 10 mM
Dithiothreitol (DTT), 10 mM
Eagle's MEM
Earle's salt solution
EDTA, 0.2 M
EGTA, 0.2 M Ethanol, 10%
Glucose, 20%
Glycerol, 5%
Glycine, 0.1 M
Guanidine-HCl, 2 M
Hank's salt solution
HCl, 0.1 M
HEPES, 0.1 M
Imidazole, 0.2 M
Magnesium chloride, 1 M
MES, 0.1 M
Methanol, 10%
Modified Dulbecco's PBS
MOPS, 0.1 M
NAD, 2 mM
Nonidet P-40, 0.25%
Octyl
b-glucoside, 0.5%
Octyl b-thioglucopyranoside, 1%
PBS
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