Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 19)

5. Incubate at room temperature for at least
5 min. Samples should not be incubated
longer than 1 hr at room temperature.
6. Set the spectrophotometer to 595 nm. Zero
the instrument with the blank sample (not
required for microplate readers). Measure the
absorbance of the standards, blanks, and
unknown samples. Refer to Section 3 for
data analysis.
Note: If the spectrophotometer has a
reference and sample holder, the instrument
can be zeroed with the blank samples. If the
effect of buffer on absorbance is required,
zero the instrument with a cuvette filled with
water and dye reagent in the reference
holder.
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