Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 7)

gamma--globulin, the listed reagents show little or
no interference.
1.2 Selecting a Protein Standard
In any protein assay, the ideal protein to use as a
standard is a purified preparation of the protein
being assayed. In the absence of such an
absolute reference protein, another protein must
be selected as a relative standard. The best
relative standard to use is one that gives a color
yield similar to that of the protein being assayed.
Selecting such a protein standard is generally
done empirically. Alternatively, if only relative
protein values are desired, any purified protein
may be selected as a standard. The two most
common protein standards used for protein
assays are BSA and gamma-globulin.
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