Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 32)

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Section 7
Appendix
5 ml Standard Assay
Standard Source of Diluent Final
Tube # Volume (µl) Standard Volume (µl) [Protein] (µg/ml)
1 300 2 mg/ml stock 0 2,000
2 375 2 mg/ml stock 125 1,500
3 325 2 mg/ml stock 325 1,000
4 175 Tube 2 175 750
5 325 Tube 3 325 500
6 325 Tube 5 325 250
7 325 Tube 6 325 125
8 (blank) – – 325 0
1 ml Standard Assay
Standard Source of Diluent Final
Tube # Volume (µl) Standard Volume (µl) [Protein] (µg/ml)
1 70 2 mg/ml stock 0 2,000
2 75 2 mg/ml stock 25 1,500
3 70 2 mg/ml stock 70 1,000
4 35 Tube 2 35 750
5 70 Tube 3 70 500
6 70 Tube 5 70 250
7 70 Tube 6 70 125
8 (blank) – – 70 0
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