LABEC M-MIA-A User Manual
Page 87
 
Ultrasonic milk analyser
reason it is always a good idea to have a back-up electrode on hand to avoid 
any system down time. 
 
3. Buffer Solutions 
Buffers are solutions that have constant pH values and the ability to resist 
changes in that pH level. They are used to calibrate pH measurement 
system. 
 
PH buffer solution description ( Pharmacopoeia standard)
Use only this types standard buffers for calibration!
 
Description 
pH 7.00±0,01/20ºC
pH 4.00±0,01/20ºC
Composition Potassium
dihydrogen
phosphate, Di-sodium 
hydrogen phosphate 
Borax, Sodium 
hydroxide solution 
Temperature 
parameters 
 
10ºC - 7.06 
25ºC - 6.99 
20ºC - 7.00 
30ºC - 6.98 
40ºC - 6.95 
50ºC - 6.91 
10ºC - 4.00 
25ºC - 4.00 
20ºC - 4.00 
30ºC - 4.00 
40ºC - 4.00 
50ºC - 4.05 
 
4. pH Electrode Calibration 
pH Electrodes are like batteries; they run down with time and use. As an 
electrode ages, its glass changes resistance. For this reason, electrodes 
need to be calibrated on a regular basis. Calibration in pH buffer solution 
corrects for this change. 
Calibration is an important part of electrode maintenance. This assures not 
only that the electrode is behaving properly but that the system is operating 
correctly. 
Usually pH meters require calibration at 3 specific pH values. One calibration 
is usually performed at pH 7, second and third are typically performed at pH 4 
and pH 10. 
 
It is best to select a buffer as close as possible to the actual pH value of the 
sample to be measured. Use standard calibration buffers that the 
temperature and the sample solution are the same. 
Use the operation manual for the corresponding pH meter. 
 
Operation manual
MRC.VER.01-02.10