Luminex 200 User Manual with IS 2.3 Software - Manual (Page 28)

Luminex 200 System User Manual
x
MAP Technology
3 - 2 PN 89-00002-00-144 Rev. B
4. Click Alcohol Flush. A confirmation dialog box opens. Click
Eject/Retract to eject the Luminex XYP instrument tray. Place
at least 1.2 mL of 70% isopropanol or 70% ethanol in the
reservoir. Click OK.
5. Click Wash. In the confirmation dialog box, click on the drop-
down arrow located to the right of the Eject/Retract button to
choose the desired location. Click Eject/Retract. The Luminex
XYP instrument tray ejects. Place distilled water in the selected
well or reservoir on the plate. Click OK. Perform this step twice.
6. Check that the Luminex XYP instrument sample probe has been
vertically aligned for the plate used in the kit.
Adjust the sample
probe vertical
height
‹
To adjust the sample probe vertical height:
1. Remove the clear plastic shield that covers the sample probe
area.
2. In a 96-well microtiter plate where overall height is no more than
0.75 inches (19 mm), place the appropriate alignment tool in the
plate:
For a standard plate with flat-bottom wells—stack two of the
larger (5.08 mm diameter) alignment discs together and place
them into the selected well.
For a filter bottom plate—stack three of the larger (5.08 mm
diameter) alignment discs together and place them into the
selected well.
For a half-volume plate with flat-bottom wells—stack two of
the smaller (3.35 mm diameter) alignment discs together and
place them into the selected well.
For a round-bottom (U-bottom) plate—stack two of the
smaller (3.35 mm diameter) alignment discs in the selected well.
For a plate with conical wells—place one alignment sphere into
the selected well.
3. Select the Maintenance tab, then click Eject/Retract to eject
the plate holder. Place the 96-well microtiter plate on the
Luminex XYP instrument plate holder with position A1 in the
top left corner.
4. Verify that the correct location is selected in the Luminex IS 2.3
Software, and that you are using the appropriate number of
alignment discs. Click Eject/Retract to retract the plate.
Note: Verify that the microtiter
plate is not warped. Warped
plates can lead to incorrect
probe height adjustment.
Note: Alignment discs can be
placed in any well as long as the
well is designated in the software.