Luminex 200 User Manual with IS 2.3 Software - Manual (Page 45)

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MAP Technology Troubleshooting
PN 89-00002-00-144 Rev. B 4 - 3
Pressurization
Normal air and sheath pressure readings vary between 6-9 psi while
the compressor runs. If the system pressure is out of range, your
sample acquisition will fail or return poor results.
Symptom Possible problem Solution
Pressurization fails or
pressure is too low. The sheath and waste lines are
not fully connected. Make sure the lines between the
sheath and waste bottles and the
analyzer are fully connected.
The sheath or waste bottle
fittings are cracked. Inspect the fittings to be sure
they form a tight seal.
There is a leak in the system. Check for system leaks. A leak
will be obvious if there is fluid
on the surface on which the
system sits.
The compressor does not
engage. Run a Prime command. If you
do not hear the compressor turn
on, contact Technical Support.
The Cheminert fitting is loose. Ensure that the fitting connects
tightly above the sample probe,
below the blue light.
Fluid leaks in the system. See Fluid Leak on page 4-4.
The sheath bottle has an air
leak. Disconnect the sheath and waste
bottle connections from the
analyzer. Run a Prime
command. If pressure builds,
remove and retighten the sheath
fluid bottle cap, then reconnect
fluid lines to the analyzer. If
pressurization fails again,
replace the sheath bottle.
Pressure too high. The sheath bottle is overfilled. Ensure that the sheath bottle is
not filled above the fill line.
The Sheath Delivery System is
overfilled. Drain the SD reservoir and refill
it. See page 4-17.
Regulator not adjusted properly. If using bottles, open the center
door on the Luminex analyzer.
Use a screwdriver to adjust the
regulator to fit in the center of
the green region on the Run
Batch tab.