Luminex 200 User Manual with IS 2.3 Software - Manual (Page 50)

Luminex 200 System User Manual
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MAP Technology
4 - 8 PN 89-00002-00-144 Rev. B
Acquisition
Problems
There is air in the system. Verify sample probe height. Run
three Prime commands, two
Alcohol Flush commands, then
three Washes with water
The waste container is not
properly vented. Verify that the waste container is
properly vented and that the seal
is not wet or soiled.
The waste line was moved
during system operation,
resulting in unstable flow rate. Verify that the waste line is not
moved during system operation.
Possible problem with the
lasers. Check the system control trend
report for consistent failures. If
any are evident on the report,
contact Technical Support.
Symptom Possible problem Solution
Acquisition fails or slows. The air pressure is out of range. See Pressurization on page 4-3.
The sample probe height is
incorrect. Adjust the sample probe height.
See page 3-2.
The sample probe is clogged. Clean the sample probe. See
page 3-5.
The sheath bottle has a leaky
seal. Make sure that the sheath bottle
lid is tightened. Remove and
replace the sheath bottle lid.
The sheath or waste lines are
not fully connected. Disconnect and reconnect the
lines; you should hear them
click.
The calibration microspheres
have expired. Replace old microspheres with a
fresh lot.
The wrong wells are selected for
the calibration microspheres. Ensure that the correct wells are
selected in the Maintenance tab.
The calibration lot number or
target values selected in setup is
incorrect. Enter the correct calibration lot
number and target values in the
Update CAL Targets box.
Slow or unsuccessful sample
acquisition. The sample probe is clogged. Clean the sample probe. See
page 3-5.