Luminex 200 User Manual with IS 2.3 Software - Manual (Page 51)

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MAP Technology Troubleshooting
PN 89-00002-00-144 Rev. B 4 - 9
Bead Detail
Irregularities
Use these tools to assist in diagnosing system and kit-related
problems:
• system calibrators
• system controls
• assay standards
• assay controls
• error messages
Review calibration/control trend reports routinely to detect trends.
Use system xMAP control microspheres to check the success of the
system calibration and for troubleshooting purposes. If there is a
problem with your kit results, xMAP controls can help determine if
the problem is analyzer related. If Calibration and Controls are
successful, contact the kit manufacturer.
The air pressure is out of range. See Pressurization on page 4-3.
The sample probe is not
vertically aligned. Adjust the sample probe height.
See page 3-2.
Air is present in the system. Verify sample probe height. Run
three Prime commands, two
Alcohol Flush commands, then
three Washes with water
The acquisition volume is set
too high. Set the acquisition volume to at
least 25 µL less than the actual
volume in your wells. This
setting lets the analyzer acquire
sample more efficiently with
less chance of acquiring air.
The xMAP microspheres are not
fully suspended. Gently vortex the plate or
resuspend beads with a
multichannel pipettor to ensure
that the microspheres are
present in the solution.
You are using photobleached
microspheres. Replace the microspheres with a
fresh batch.
There is an insufficient number
of beads in the sample. Ensure that there are 2000-5000
beads per beadset per well.
The sample is too concentrated. Dilute concentrated biological
fluids, such as serum or plasma,
at least 1:5.