Luminex 200 User Manual with IS 2.3 Software - Manual (Page 49)

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MAP Technology Troubleshooting
PN 89-00002-00-144 Rev. B 4 - 7
Zero events collected during
calibration. There is a problem with fluid
levels. Check the sheath and waste
fluid levels. Verify that tubing
for both bottles is tightly
connected to the instrument.
Check that the waste bottle cap
is vented.
Laser-related issue. Verify fluid is moving through
the system by performing a
Wash, which causes fluid to go
out to waste. If no fluid is going
to waste, clean the sample
probe, then flush the system
(see page 3-5). If the issue does
not resolve, contact Technical
Support.
The waste container is not
vented. Check that the seal in the waste
container cap is dry and that the
waste container cap is vented.
The Cheminert fitting is loose. Make sure that the Cheminert
fitting is tightened.
Analyzer fails Controls. The control microspheres are
not fully suspended. Vortex the control vials to
resuspend the microspheres.
Wrong control lot number or
target values are entered in
Update CON Targets box. Verify that the correct lot
number and target values are
used.
The system controls are in the
wrong well on the plate. Verify that control microspheres
are in the correct well.
Not enough control
microspheres added to the well. Add at least five drops of control
microspheres to the well. For
accurate drop volume, hold the
vial upside down at a 90 degree
angle to the plate while
dispensing them.
Control lot is expired. Use a fresh bottle of control
microspheres.
Control microspheres have been
diluted. Do not dilute the control
microspheres.
The sample probe height is
incorrect. Adjust the sample probe height.
See page 3-2.
The sample probe is clogged. Clean the sample probe. See
page 3-5.