Luminex 200 User Manual with IS 2.3 Software - Manual (Page 48)

Luminex 200 System User Manual
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MAP Technology
4 - 6 PN 89-00002-00-144 Rev. B
Calibration and
Control Problems
Symptom Possible problem Solution
Calibration is slow or fails. The calibration microspheres
are not fully suspended. Vortex the calibration vials to
resuspend the microspheres.
Wrong lot number or target
values are entered in Update
CAL Targets dialog box. Verify that correct lot number
and target values are used.
The system calibrators are in the
wrong well on the plate. Verify that calibrators are in the
correct well.
Not enough calibrator
microspheres added to the well. Add at least five drops of
calibrator microspheres to the
well. Hold the vial upside down
at a 90 degree angle to the plate
while dispensing them.
Calibrator lot is expired. Use a fresh bottle of calibrator
microspheres.
The sample probe height is
incorrect. Adjust the sample probe height.
See page 3-2.
The sample probe is clogged. Clean the sample probe. See
page 3-5.
There is a partial clog in the
system. Clean the sample probe, then
flush the system. See page 3-5.
There is air in the system. Verify sample probe height. Run
three Prime commands, two
Alcohol Flush commands, then
three Washes with water.
The waste container is not
vented. Check that the seal in the waste
container cap is dry and that the
waste container cap is vented.
The waste line was moved
during system operation,
resulting in unstable flow rate. Verify that the waste line is not
moved during system operation.
Possible problem with the laser. View the calibration trend
report. Check for dramatic
changes in temperature, sheath
pressure, or voltages. If any of
these are evident on the report,
contact Technical Support.