Multichannel Systems Roboocyte2 Manual - Manual (Page 103)

Analysis with Roboocyte2+
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Result Display
On the right side of the lower part you can choose in the "Result Display" whether the recording
results are calculated as "IV-Curve" or as "Dose-Response-Curve".
Displaying and Fitting of Dose-Response Data
To use the feature of plotting and fitting dose-response curves in the “Result Display” window,
two conditions have to be fulfilled already during recording the data:
 Compounds and their respective concentrations have to be defined for the valves used and the
respective liquid configuration has to be selected. See chapter "Linking Compound Applications
to Recorded Data" for details.
 The recording script has to contain the two commands
Command 1: Robo2.TransmitRecording(REC_TAG_REF_COMPOUND);
or Robo2.TransmitRecording(REC_TAG_ COMPOUND);
Command 2: Robo2.TransmitCompoundValve(valve);
where "valve" is the variable for the valve number.
Robo2.TransmitRecording(REC_TAG_REF_COMPOUND); will cause the data to be plotted in the
“Dose-Response” tab of the “Result Display”, but it will not be used for fitting. This is for example
useful for the application of a reference concentration to check the expression before starting the
full dose-response protocol.
Robo2.TransmitRecording(REC_TAG_ COMPOUND); will also cause the data to be plotted but the
data will also be used for fitting a dose-response curve. Robo2.TransmitCompoundValve(valve);
is used in Roboocyte2+ to generate the concentration axis of the dose-response-plot.
The two commands have to be "inside" of a recording, for example between
Robo2.StartRecording(); and Robo2.StopRecording();
Example
Robo2.StartRecording();
Robo2.TransmitRecording(REC_TAG_REF_COMPOUND);
Robo2.TransmitCompoundValve(reference);
Robo2.Wait(pre_agonist_s);
Robo2.OpenValve(reference);
Robo2.Wait(agonist_s);
Robo2.OpenValve(1);
Robo2.Wait(10);
Robo2.StopRecording();