Multichannel Systems Roboocyte2 Manual - Manual (Page 76)

Roboocyte2 Manual
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7.2 Alignment
You have to align the position of the carrier relative to the measuring head i.e. the electrode tips
under microscopic control. The Roboocyte2 always moves relative to a standard position. The aim
of the alignment process is to define this standard position. If this is not done properly, the
Roboocyte2 moves to wrong positions. The alignment process also compensates for minor
changes in the dimensions of different well plates and variations in the length of the electrodes.
To achieve a stable recording, it is essential to perform an alignment each time before you start
a recording sequence.
Note: The alignment is very important for obtaining best results. An alignment is strongly
recommended each time before you start recording. It is very important that the glass capillaries
are centered on the alignment device. Otherwise, the oocytes are not properly hit.
Warning: Hitting the alignment device can cause the glass capillaries to shatter, possibly
projecting splinters, which can be dangerous. Eye protection should be used, and this alignment
should never be observed up close with an unprotected eye.
Place the microscope in a position onto the carrier table which allows to focus on the alignment
device plugged in well H12.
Alignment Device
Click "Alignment Wizard" in main menu "Tools". Or click the icon in the main window.
The "Preparation" window of the "Alignment Wizard" dialog appears.
Click "Change Plate Pos" to install the alignment device on well H12.