Multichannel Systems Roboocyte2 Manual - Manual (Page 56)

Roboocyte2 Manual
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The upper section consists of command buttons for movements, amplifier controls before
impalement and valve and pump control buttons. Use "Go to Well" to move the carrier to the
selected well and "Home" to move it back to home position. Clicking the button "Liquid" moves
the z-axis down to a well, for example into the solution of the well. Clicking the button "DC
Offset" performs a voltage offset compensation on both electrodes and is active only when a
recording in "Current Clamp" mode is started. Click the button "Clear Offset" to cancel the
offset compensation. This is useful to see the real electrode offset of both electrodes. Click the
"Z-Up" button to bring the z-axis back to the zero-position. Click "Oocyte" button to move the
z-axis, for example the electrodes down to impalement position (by default 800 μm above the
well plate bottom). If the initial impalement movement ("Oocyte") is not sufficient to impale
the oocyte, the arrow keys can be used to move further down in 50 μm steps. Finally, the twelve
valves can be individually switched on and off and the peristaltic pumps can be stopped and
started by clicking the respective buttons "Light".
Continuous Recording Window
Continuous recordings will be displayed in the recording window, the current trace in red and the
voltage trace in blue. To set the recording parameters please use the control windows right beside
of the recording window.
In the "Clamp" section, you can switch between "Current" and "Voltage" clamp mode. In the
"Set Clamp" window you define the "Holding Current" in nA or the "Holding Voltage" in mV
via up- down boxes. Click the "Set" button to confirm the clamp settings.
In the "Amplifier Control" window you adjust the amplifier parameters. Please refer to the
chapter "TEVC Recording Background" to find out more about how to optimize gain (P and I,
default 1000 and 100, respectively) and capacitive compensation (CX default 9000) of the digital
TEVC amplifier.
Choose the "Recording Frequency" in Hz, this is the sampling rate.
Clicking the "Start" and "Stop" button to start or stop a recording, if the" Record" button
is active the recorded data will be saved to the actual plate file.
Note: A recording can only be saved when a plate file is loaded. If no plate is loaded,
the "Record" button is grayed out and cannot be activated.