Multichannel Systems Roboocyte2 Manual - Manual (Page 84)

Roboocyte2 Manual
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6. Stop the current clamp recording and start the recording under voltage clamp by selecting
"Voltage" instead of "Current" and press "Start".
The display will show the voltage of -60 mV (if -60 mV is selected) and the corresponding current
the size of which is dependent on the membrane resistance of the oocyte, for example only tens
of nanoamps for a "good oocyte".
Check membrane current with perfusion. Start the perfusion in this sequence a. start the waste
pump b. open the valve c. start the valve pump.
Usually, the holding current gets smaller when the perfusion is on; see figure above, where the
perfusion was started at about t = 36 s. Whenever you want to save the actually recorded data,
press the "Record" button. The appearance of the red light shows that recorded data are saved
Switch between different solutions to activate, inhibit, modulate or deactivate endogenous or
expressed ion channels, receptors or transporters. The figure shows activation and deactivation
of the P2X2 receptor (an ATP-gated channel) with 3, 10, 30, 100, 300 and 1000 μM ATP.