Multichannel Systems Roboocyte2 Manual - Manual (Page 77)

Recording with Roboocyte2 Control Software
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Click next to open the "Alignment" window and the carrier will move to the x/y coordinates of
the last alignment position. At the same time the z-axis will move down to the z-position 3 mm
above the last alignment position.
Place the microscope in a position onto the carrier table which allows you to focus on the crosshair
engraved into the surface of the alignment device.
!
Move the z-axis and the carrier until the electrode tips approach the cross-hair by pressing the
respective arrow keys. Alternatively, you may use the according buttons on the keyboard. Page up
and page down keys for the z-axis and the four arrow key for the carrier. Fine tune the position
until the alignment device is almost touched by the tip of the electrodes. If you do this the first
time, you may have difficulties seeing the TEVC probe in three dimensions.
It may appear to be centered correctly, but instead the probe is still a way above the alignment
device and shifted to the front. It is very important that the capillaries are centered correctly on
the cross-hair, but the height of the tips is not so important. It is recommended to lower the TEVC
probe as low as possible without touching the surface of the alignment device, because the glass
electrodes are not so flexible and thus tend to break easily. A stepwise movement is part of the
automated recording sequence and achieves a gentle and reliable impalement with both
electrodes.