Multichannel Systems Roboocyte2 Manual - Manual (Page 37)

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4 Roboocyte2 Software
4.1 Introduction
Operate the Roboocyte2 robot, collect and evaluate the data by using the Roboocyte2 and
Roboocyte2+ software. The easy-to-use graphical user interface of the Roboocyte2 software
makes daily work with the Roboocyte2 quick and easy. Recording is started by a single mouse
click. The Roboocyte2 controls the run for all 96 oocytes automatically, even including a wash
cycle. Thus the recording can go on overnight, unsupervised.
The large amount of data generated by the Roboocyte2 can be managed with a Microsoft
Access 2010 database (not included). Multiple users can access the database over a network.
The high degree of automation and flexibility for such a demanding task is made possible by
using scripts, small text files containing commands. Users can write their own scripts with any
basic text editor or with the editor embedded in the Roboocyte2 program. All experimental
settings are defined within the script. You can write a script for any kind of experimental setup.
Once the appropriate script is loaded into the Roboocyte2 software, simply click the "Run" button
to start the robot. The script is then performed without the need for further customization and
supervision.
The Roboocyte scripting is based on Java Script with many commands specifically designed for
the Roboocyte2. It allows the full automation of various applications. For further information
on the "Java Scripting Language", please refer to the separate Java Scripting Language manual.
Although, the main use of the Roboocyte2 will be automated recording, there is a "Manual
Mode" option with which recordings can be performed on single oocytes in a non-automatic
fashion. Although, all functions will be still controlled by the Roboocyte2 software, single steps
such as impalement, clamping the cell, performing solution changes etc. are under real-time
control by the user.