Multichannel Systems Roboocyte2 Manual - Manual (Page 71)

Compound Application
67
GX-271 Rinse and transfer port station.
Important: Please note that only the leftmost rinse, drain, and transfer ports can be used with
the Gilson GX-271 liquid handler. The other transfer ports have to be closed with plugs, you can
use convenient screws. Do not remove the plugs; otherwise, the suction will not suffice to
aspirate solution from the transfer ports.
Using the transfer ports
The lines should be primed with Ringer's in the Liquid Handling Program before starting the
recording protocol. The transfer ports can also be used for applying larger volumes of solutions
during the protocol.
Important: In the script, use the appropriate Gilson.Valve1On(); or Gilson.Valve2On(); commands
to open the transfer port lines 1 or 2, respectively. If the valve is closed, liquid aspiration is not
possible. See the Roboocyte2 Scripting Language manual for more information.
1. Put the tube leading to transfer port 1 into reservoir 1, for example, a bottle with frog Ringer
and the tube leading to transfer port 2 into reservoir 2.
2. Move the Gilson needle into the appropriate transfer port 1 or 2 by using the respective buttons
in the "Liquid Handling" tab of the "Manual Mode".
3. Open the solenoid valve of the appropriate transfer port 1 or 2 by using the respective buttons
in the "Liquid Handling" tab of the "Manual Mode".
4. Switch on the Minipuls 3 pump in backward direction by using the respective buttons
in the "Liquid Handling" tab of the "Manual Mode".