Multichannel Systems Roboocyte2 Manual - Manual (Page 80)

Roboocyte2 Manual
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Gilson Liquid Handler
When using the Gilson Liquid Handler, the manifold will not be used and the tubing coming from
the Gilson peristaltic pump is directly connected to the inflow of the measuring head. Therefore,
this tubing will be the only one that has to be primed before use. Similar to the prime procedure
when using the Roboflow, you can use again the "Liquid Handling" tab of the "Manual Mode"
window.
In order to work with the Gilson liquid handler, be sure that you have selected "Gilson
Connected" in the "Options" of the main menu "Settings".
In addition you have to select a "Liquid Configuration" for the Gilson in main menu "Tools",
"Define Liquid Configurations". Please use the predefined liquid configuration "Prime" for
priming from one of the two transfer ports (T-Ports).
Put the transfer port tubing into an appropriate solution, move the Gilson probe (needle) to
transfer port 1 or 2, open valve 1 or 2 and switch on the Gilson pump in backward direction.
Wait until the tubing is completely filled and no air bubbles are left. Priming is completed.