Multichannel Systems Roboocyte2 Manual - Manual (Page 69)

Compound Application
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6.2 Using the Roboflow System
The Roboflow-System is simple, easy to maintain, and ideally suited for most rapid standard and
expression tests, dose response analyses, and small screens. The system consists of twelve valves
and two peristaltic pumps. The perfusion can be continuous, but you can also pause the perfusion
during the recording. The small volume of a well ensures that a flow rate of about 4 ml/min
achieves a rapid and efficient solution exchange with effective compound usage. You can easily
implement your own automated drug-saving strategies into your experimental setup.
The movable peristaltic pump is connected to the measuring head inlet and is for compound
application. The peristaltic pump fixed on the upper right side of the front panel of the Roboflow
device is for aspiration to the waste bottle. The endings of the perfusion inlet and outlet tubings
are fixed to the measuring head at different heights, which keeps the fluid level constant.
You can either control the opening of the valves as well as the speed of application manually
during manual recording or use the designated commands in a script. For details, refer to the
Scripting Language Manual.
The definition of compounds and buffer for the Roboflow-System is in principle the same as
in other liquid handlers. It is described in the next chapter "Using the Gilson Liquid Handler".
6.3 Using the Gilson Liquid Handler
The liquid handling of the Roboocyte2-System is possible either with the Roboflow-System from
Multi Channel Systems MCS GmbH or with the liquid handler GX271 from Gilson. To connect one
of these systems, please read chapter “Setting up the Roboflow-System” and / or “Setting up the
Gilson GX-271”.
If the Gilson Liquid Handler is used, you have to activate the communication with the GX-271
in the "Options" dialog box. Please click main menu “Settings” and select “Options”.
Activate the check box “Gilson Connected” and restart the software after switching the Gilson
on. During the software starts, the GX-271 will be initiated and will perform its reference
movements.
Important: After changing the selection in the “Gilson Connected” check box, you have to exit
the Roboocyte2 program and start it again.