3 connection of the injection valve, 4 mounting the column – Metrohm 817 Bioscan User Manual

Page 24

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2 Installation

817 Bioscan

16

2.3.3

Connection of the injection valve

Connect all capillaries to the injection valve according to the following
description:

2

1

4

5

3

6

42

43

44

47

46

45

Fig. 5: Connections at the injection valve of 2.812.0010 valve unit

42

Connection for syringe tubing
PTFE tubing with coupling
(6.2744.120) and syringe (6.2816.020,
without needle)

45

Eluent outlet
Connection to preheating capillary

31

(PEEK, 6.1836.010)

43

Connection for aspirating tubing
PTFE tubing for aspirating sample

46

Eluent inlet
PEEK capillary connection

29 to

pulsation absorber

44

Connection for sample loop
20 µL PEEK sample loop (6.1825.210)

47

Connection for sample loop
20 µL PEEK sample loop (6.1825.210)

2.3.4

Mounting the column

The IC separating column (e.g. Metrosep Carb 1, 6.1013.000) is first
mounted in the 817 Bioscan as follows (see Fig. 3):

1 Connect column to the preheating capillary

• Remove the caps from column 25.
• Screw eluent outlet of preheating capillary 31 on to the inlet

end of the column (observe flow direction) .

2 Connect column to flow-through measuring cell

• Screw outlet end of column 25 on to eluent inlet 41 of the

flow-through cell using the 6.2744.130 KELF pressure screw.

3 Fix column

• Fix the column in column holder 26.

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