Metrohm 817 Bioscan - Manual (Page 63)

5.1 Practical notes on ion chromatography
817 Bioscan
55
5 Notes – Maintenance – Faults
5.1 Practical notes on ion chromatography
5.1.1 Separating columns
Separation efficiency
The attainable quality of analyses with an IC system depends to a large
extent on the separation efficiency of the column used. When purchas-
ing an IC column you should ensure that the separation efficiency suf-
fices for the analysis problems at hand. Compare the standard chro-
matogram enclosed with the column with your own measurements. If
any difficulties arise, you should always first check the quality of the
column by recording a standard chromatogram.
You will find additional detailed information on the separating columns
available from Metrohm in the leaflets supplied and in the Application
Works and -Notes, which are available on request free of charge at
your local Metrohm agency.
Protection
To protect the column against foreign particles which could have an
adverse influence on the separation efficiency, we advise you to subject
both the eluents and all samples to microfiltration (0.45
µm filter) and
to siphon the eluent through the 6.2821.090 Aspirating filter.
To avoid contamination by abrasive particles arising from piston seals
of the high-pressure pump, it is advantageous to install an in-line filter
between the pump and the injection valve. The 6.2821.100 Filter unit
PEEK is best suited for this purpose. It is a part of the standard equip-
ment of the 709 IC Pump.
Storage
Always store the separating columns closed and filled when not in use
in accordance with the manufacturer’s specifications.