Metrohm 817 Bioscan - Manual (Page 27)

2.3 Capillary connections
817 Bioscan
19
2.3.7 Rinsing the tubing
The pulsation absorber is supplied permanently mounted and is filled
with isopropanol. In order to remove this and any air that may be pre-
sent in the capillaries and the measuring cell all the eluent-carrying tub-
ing or capillary connections must first be installed; the column and the
flow cell must not be connected during the beginning of the process.
Proceed as follows:
1 Deconnect preheating capillary from column
• Deconnect the eluent outlet of preheating capillary 31 from
the column and fix it at a waste bottle.
2 Set injection valve to “Inject”
• Press the <INJECT> key on the 812 Valve unit, which should
not yet be connected to the 817 Bioscan. The green LED in
the key lights up to show that the injection valve is in the “In-
ject” position.
3 Vent 709 IC Pump before start up
• Proceed according to Section 2.7 of the ‘Instructions for Use’
of the 709 IC Pump.
4 Rinse with dist. H
2
O
• Immerse the aspiration capillary of the 709 IC Pump in dis-
tilled or deionized water.
• Switch on the 709 IC Pump, rinse the IC system for approx.
10 min. and switch off the 709 IC Pump.
5 Rinse with eluent
• Immerse the aspiration capillary of the 709 IC Pump in the
eluent to be used (e.g. 100 mM NaOH).
• Switch on the 709 IC Pump, rinse the IC system for approx.
10 min. and switch off the 709 IC Pump.
6 Connect flowcell and rinse with eluent
• Connect the preheating capillary 31 to flowcell using column
connection capillary 21 and 6.2620.060 Coupling.
• Switch on the 709 IC Pump, rinse the IC system for approx.
10 min. with eluent and switch off the 709 IC Pump.
7 Remove the coupling
• Remove the 6.2620.060 Coupling between column connec-
tion capillary 21 and preheating capillary 31.
• Remount the column between column connection capillary
21 and preheating capillary 31 as show in Fig. 3.
2.3.8 Rinsing the column
In order to rinse or precondition the column the connection capillary 21
to flowcell must be unscrewed from the column. Proceed as described
in point 3 and 4 in section 5.2.4.