Metrohm 817 Bioscan - Manual (Page 40)

2 Installation
817 Bioscan
32
2.6 Preparing the 817 Bioscan for the analysis
This section describes the necessary steps for preparing the 817 Bio-
scan to carry out an analysis. A more detailed description of the opera-
tion of the 817 Bioscan using IC Net 2.1 can be found in Section 3 and
in the ‘Instructions for Use’ of the 8.110.8221 Metrodata IC Net 2.1
software.
2.6.1 System switch-on
1 Mains on
Start up the individual instruments and the IC Net program in the
following sequence:
1. Computer
2. IC instruments
3. IC Net 2.1
2 Check measuring cell
For safety reasons check the status of the measuring cell in the
instrument window of the Bioscan (accessed by a double-click
with the mouse on the instrument symbol) under
Manual / Cell .
If the 817 Bioscan is switched on without the flow-through
measuring cell being constantly rinsed through a conducting
eluent (e.g. 0.1 M NaOH) or correctly connected up then the
measuring cell must not be switched on!
3 Check the external control of the 709 IC Pump
Depending on the control of the 709 IC Pump (MIC-8: manual;
combination with 762 IC Interface: external) the setting of the
external control of the IC Pump should be checked with key
8
<EXT.>.
2.6.2 Start pump
1 Column/ Eluent
With the Metrosep Carb 1 (6.1013.000) column use 0.1 M NaOH
(quality) as the eluent for sugar analysis. This should be made
up and degassed freshly every day; it should at least be de-
gassed before use each day.
2 Flow rates / Pressure
The Metrosep Carb 1 column should not be subjected to large
variations in pressure. For this reason each time that the eluent
is used it should be pumped for a period of 10 minutes both
before and after use at 0.3 mL/min. For normal operation a flow
rate of1.0 mL/min is used.
Set a maximum pressure P
max
of 15 MPa. At 1 mL/min and with
0.1 M NaOH this should establish a pressure of approx. 9 - 10
MPa with the Metrosep Carb 1.