7 photometry – ALTANA LCS III User Manual

Page 56

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Standard Operations

5.7 Photometry

In the photometry mode, photometric measurements can be carried
out as:

Single wavelength measurements

Multi wavelength measurements

Time course

Wavelength Scans

5.7.1 Single Wavelength (absorbance, concentration and transmittance measurements)

The Single Wavelength mode can be used in three ways. For
sample measurements at a single wavelength, the instrument can
be programmed to measure the absorbance, % transmittance or
concentration of the analyte.

Absorbance measures the amount of light absorbed by the sample,
in units of Absorbance.

% transmittance measures the percent of the original light that
passes through the sample and reaches the detector.

Turn the concentration factor on to select a specific multiplier for
converting absorbance readings to concentration. In a graph of
concentration versus the absorbance, the concentration factor is
the slope of the line.

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