2 time course scan reading, Standard operations – ALTANA LCS III User Manual

Page 70

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Standard Operations

Scale & units:

1.

Press Scale & Units in the Options menu.

2.

Highlight Abs or %T as the required units.

3.

Highlight Auto or Manual scaling on the graph´s y-axis.

Note: If manual scaling is selected, use the alphanumeric keypad to set the
limits y

min.

and y

max.

. The graph is adjusted to display only the values in the

selected range. If automatic scaling is selected, the instrument sets the
limits automatically so that the total range can be displayed.

4.

Press OK to confirm.

5.

Press Return to return to the scan mode.

5.7.4.2 Time course scan reading

After the parameters have been selected, the instrument must be
blanked, then the sample can be analyzed.

1.

Insert the blank cuvette/cell into the cuvette/sample cell holder.
Press Zero. The blank reading is shown on the display.

2.

Insert the sample cuvette/cell into the cuvette/sample cell
holder. Press Read. Start collecting time course (kinetic) data.

Note: During the measurement the

Zero

and

Read

keys change to

Mark

and

Stop

.

Select Mark to mark the next data point collected. This mark is
not used by the instrument, but is available for the user and
may indicate a significant event, such as the addition of a
sample or other reagent. The mark is also shown in the table.

Select Stop to end the sample readings.

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