ALTANA LCS III - Manual (Page 68)

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Standard Operations
Second option:
1. Insert the blank cuvette/cell into the cuvette/sample cell holder.
Press Zero.
2. Insert the sample cuvette/cell into the cuvette/sample cell
holder. Press Read.
• The newly plotted wavelength scan curves are shown in
black.
• The absorbance or transmittance value and the associated
wavelength are highlighted in black.
3. Press Options and then Reference: Off in the Options menu
to select another scan to display on the same screen with the
current scan. Highlight the required scan number and press
Highlight Reference.
Note: After selecting a reference scan the Reference: Off key in the
Options menu turns into Reference: On.
Note: Only scans that have the same wavelength range and step can
be displayed using the overlay option. This process can be repeated
until all matching scans are displayed.
4. The reference curve is shown in orange. The absorbance or
transmittance value and the associated wavelength are
highlighted in orange.
• Additionally the difference of the extinction and/or
transmission value between the two Scans (measured
Scan and reference Scan) is indicated/highlighted at each
position of the cursor.
Note: A black and an orange box are shown in the left corner of the
display. The orange box relates to the reference scan and the black
one relates to the current wavelength scan.
5. Press the black or orange small box in the left upper corner on
the screen to switch between the actual wavelength scan and
reference scan.
5.7.4 Time course of absorbance/transmittance
The Time Course Mode is used to collect data in either absorbance
or transmittance for a user-specified length of time. After the data
are collected, they can be displayed in either graphic or tabular
format.
5.7.4.1 Time course setup parameters
Press Time Course mode in the Main Menu. Press Options to
configure parameters.
Table 11 describes the configure parameter
options.