Jenway Genova Nano German - Manual (Page 19)

9. Deviations from the Beer-Lambert Law may occur at high and low concentrations giving non-linear
response during sample concentration measurements. For all new methods a linear range should be
defined by the preparation of a calibration curve. The quantitation mode may be used to construct such
a curve against which sample results are automatically measured.
10. Cuvettes and sample holders must be filled to a minimum level which covers the light path. All
Jenway spectrophotometers have a beam height of 15mm.
11. The instrument must be calibrated to zero absorbance/100% transmittance prior to taking readings.
In the spectrum measurement mode a baseline scan must be performed before performing a sample
scan.
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