Jenway Genova Nano German - Manual (Page 77)

16.2.2.4 Setting the Dilution Factor
In the settings menu press the key adjacent the dilution
icon. This screen allows the user to input the relative sample
and diluent volumes which have been used to prepare
the sample being measured. Using this information, the
instrument calculates a dilution factor which allows the
instrument to determine and display the concentration of
the original undiluted sample.
The volume of sample is entered by pressing the key
adjacent to the sample volume icon. This will open the
number entry screen. Use the keys at the bottom of the
screen to select the digit to be changed and use the keys
adjacent to the arrow icons to increase or decrease the
number. The value can be reset to one by pressing the key
adjacent to the 001 icon. Once the required volume has
been entered press the key adjacent to the tick icon to save
the changes and return to the settings.
The volume of diluent is entered by pressing the key
adjacent to the diluent volume icon. This will open the
number entry screen. Use the keys at the bottom of the
screen to select the digit to be changed and use the keys
adjacent to the arrow icons to increase or decrease the
number. The value can be reset to zero by pressing the key
adjacent to the 000 icon. Once the required volume has
been entered press the key adjacent to the tick icon to save
the changes and return to the settings.
After the sample and diluent volumes have been entered
the dilution factor screen will show a summary of the
dilution information entered.
16.3 CALIBRATION
In the OD 600 measurement mode calibrations against a
standard or a factor can be performed following a zero
calibration. If the factor is not known calibration against
a known standard is performed in order to calculate the
factor. However if the factor is known there is no need to
calibrate using a standard.
The calibration must be performed at the same wavelength at which the sample will be measured.
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