Jenway Genova Nano German - Manual (Page 73)

15.6 BIURET ASSAY
The Biuret operating menu enables the user to perform
the Biuret protein assay at the specified wavelength of
540nm.
The Biuret settings menu allows the user to adjust
measurement specific parameters such as the number of
standards and result resolution. Other method settings
can be altered if required, but the Biuret method settings
are locked and any changes will be lost after exiting the
mode. See Section 8 for full instructions on altering the
Biuret menu options, settings and creating a new standard
curve.
15.7 DIRECT UV
The direct UV operating menu enables the concentration
and purity of a protein sample to be estimated according
to the following equations:-
Purity Ratio = Abs@280nm / Abs@260nm
Conc(μg/ml) = (Abs@280nm x 1550) - (Abs@260nm x 760)
The Direct UV settings menu allows the user to adjust
measurement specific parameters such as the dilution factor
and result resolution. Other method settings, such as the
inclusion of a reference wavelength and the concentration
equation/factors can be altered if required, but the Direct
UV method settings are locked and any changes will be
lost after exiting the mode.
See Section 13 for full instructions on altering the Direct UV method options and settings.
15.8 CALIBRATION AND SAMPLE MEASUREMENT
When adjustments to the selected methods measurement parameters are complete insert a cuvette
containing the blank solution into the sample chamber and close the instrument lid. Press the key below
the calibrate to zero absorbance icon.
Remove the cuvette containing the blank solution and place a cuvette containing the sample to be
measured in the sample chamber. Close the instrument lid and press the key below the measure sample
icon. Once the measurement is complete the screen will show a summary of the results.
Operating Menu
Operating Menu
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