Jenway Genova Nano German - Manual (Page 44)

Repeat presses of the key cycles the curve fit between
1. Linear Regression Concentration = Abs x A + B
2. Linear Regression Through Zero Concentration = Abs x A
3. Quadratic Regression Concentration = A x Abs 2 + B x Abs + C
4. Quadratic Regression Through Zero Concentration = A x Abs 2 + B x Abs
5. Interpolate Concentration(n) = Abs x A(n) + B(n)
The curve statistics can be displayed by pressing the
key below the S icon; this opens a screen on top of the
existing curve displaying statistics for the curve fit chosen.
For example if the curve fit is Conc = Abs x A + B the
curve statistics displayed will be the gradient of the line
(A), constant (B) and correlation coefficient (r
2 ). To exit
this screen press the key adjacent to the tick icon. The
quantitation table can also be viewed from this screen by
pressing the key adjacent to the quantitation table icon.
A new standard curve can be created by pressing the key below the measure standards icon. See section
8.2.2.2 for details on creating a new standard curve.
Once the curve has been created press the key adjacent to the tick icon and the screen will return to the
operating menu where unknown samples can be measured.
8.3 CALIBRATION
The zero calibration must be performed at the same
wavelength as the standards were measured at and the
unknown samples will be measured at. Insert a cuvette
containing the blank solution into the sample chamber and
close the instrument lid. Press the key below the calibrate to
zero absorbance icon. The instrument will calibrate to zero
absorbance. Once the calibration is complete the measure
sample icon appears and the sample can be measured. If
the wavelength is adjusted before a sample is measured the measure sample icon will disappear and the
instrument must be calibrated again at the new wavelength.
8.4 SAMPLE MEASUREMENT
Once a calibration has been performed the measure
sample icon appears. A standard curve should either be
created or loaded from a previously saved method before
carrying out a measurement of an unknown sample. Place
the cuvette containing the sample in the sample chamber
and close the instrument lid.
Press the key below the measure sample icon and the instrument will take a reading and the concentration
and photometric values will be displayed on the screen. To view the position of this sample on the
standard curve open the standard curve screen.
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