Jenway Genova Nano German - Manual (Page 67)

SECTION 14 – DNA
The DNA measurement mode allows the user to select a method from a list of common nucleic acid
measurement tests, including single wavelength concentration measurements for dsDNA, ssDNA, RNA
and Oligonucleotides and methods that use absorbance ratios for estimating nucleic acid purity, such as
260nm/280nm and 260nm/230nm.
14.1 DNA MENU OPTIONS
The DNA menu allows the user to select the required
pre-defined method from the list of on-screen options by
pressing the key adjacent to the measurement method that
is required. The dsDNA, ssDNA, RNA and Oligo options
are used to determine the concentration of nucleic acid
samples and the 260/280, 260/230 and VarRatio methods
are used to assess both the purity and concentration of
nucleic acids.
14.2 dsDNA
The dsDNA operating menu enables the concentration of
double stranded DNA to be determined according to the
equation:-
Concentration (μg/ml) = Abs@260nm x 50.0.
The dsDNA settings menu allows the user to adjust
measurement specific parameters such as the dilution
factor and resolution. Other method settings can be altered
if required, but the concentration units are limited to µg/ml
and mg/ml only.
The dsDNA method settings are locked and any changes
will be lost after exiting the mode. See Section 11 for full
instructions on altering the dsDNA menu options and
settings.
14.3 ssDNA
The ssDNA operating menu enables the concentration of
single stranded DNA to be determined according to the
equation:-
Concentration (μg/ml) = Abs@260nm x 37.0.
Operating Menu
Menu Options
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