Leica Biosystems ASP300 S - Manual (Page 71)

72 Leica ASP300 S
6.4 Typical fill or drain problems
Fill and drain problems may be due to several causes:
1. There is insufficient reagent
• Verify that the fill level of the reagent containers and the paraffin
reservoirs is sufficient.
2. Insufficient pressure or vacuum
The instrument is unable to create sufficient vacuum (for a fill) or pres-
sure (for a drain) in the retort.
• Verify that the reagent container in question is correctly inserted in
the reagent cabinet.
• Verify that all reagent containers are fully pushed home into their
sockets.
• Check whether there is some debris under the retort lid seal caus-
ing a leak.
3. There is a blockage in the air or reagent lines
Blockages in the reagent lines are typically caused by paraffin or tis-
sue debris. If there is reagent in the retort that cannot be drained, try to
remove the blockage as follows:
• Heat the retort to the maximum possible temperature (consistent
with the reagent currently in the retort, if any). Leave the retort at
this temperature for at least 15 minutes.
• While the retort is at maximum temperature, attempt to fill or drain.
• If there is no reagent in the retort:
Run the extended retort cleaning program. Use the maximum set-
tings for number of clean cycles and temperature (consistent with
the type of reagents/solvents used in the clean program).
6. Troubleshooting
If you cannot eliminate the problem by carrying out the above listed
steps, call Leica Technical Service before attempting to run any
further programs.