Leica Biosystems RM2265 - Manual (Page 45)

43 Leica RM2265
5.9 Changing the specimen or interrupting sectioning
• Center the handle of the handwheel.
• Use the TRIM/SECT key to select the sectioning mode.
• Adjust the appropriate sectioning thickness or verify the selected value.
• Depending on the operation planned, use the CUT MODE key to select
one of the motorized sectioning modes, CONT, SINGLE or STEP.
5. Operation
5.8 Sectioning
+
Always use a different area of the cutting edge for trimming and sectioning. To do so, laterally
displace the blade or knife in the knife holder.
When using the knife holder E with lateral displacement, it is sufficient to move the knife
holder sideways.
Lock the handwheel and cover the knife edge with the knife guard prior to any manipulation of
knife or specimen head, as well as prior to changing the specimen block and during all work
breaks!
• Raise the specimen to the upper end position and activate the mechani-
cal handwheel lock.
• Cover the sectioning edge with the knife guard.
• Remove the specimen from the specimen clamp and mount a new sam-
ple to continue.
• Before cutting into a new specimen, move the specimen head back to
the rear end position.
Always set the sectioning speed according to the hardness of the
specimen! For hard specimens, always select a slow speed.
• Check the sectioning speed setting and set an appropriate speed.
• Start motorized sectioning.
• Pick up the sections and mount them on microscope slides.
Always turn the handwheel evenly in clockwise direction; other-
wise, the brake will not work properly.