Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 15)

3. To attach the filter holder to the lens of the camera, slide the filter holder up over the
lens until stops. Using the 0.9 mm hex wrench, gently tighten the setscrews on the
side of the filter holder.
Note: Bandpass Filter
The bandpass filter is used for both the white light (visible stain) and UV light (fluorescent
stain) modes. Once it is installed, it is not taken off the lens. The only adjustment to the
camera between the two light modes is a change in the aperture from the nearly closed
(f-stop 11–16) position for white light, to the open (f-stop 1.4–2.8) position for UV lights.
• Attach the camera to the camera arm by placing the camera against the ridge of the
camera mount. Secure the camera to the mount with the camera screw through the slot
in the camera mount.
2.6 Camera Lens Aperture Settings
The aperture setting on the lens is a black ring on the lens with a small black thumbscrew
attached. There are aperture settings marked on two sides of the lens. The white mark on the
ring indicates the aperture setting. F-stop setting 1.4 is maximum open for the lens; f-stop 11-16
is the maximum closed for the lens (16 is not marked but is past 11). See Figures 8 & 9 for open
and closed aperture settings. To adjust the aperture setting, loosen the thumbscrew, move the
ring to the desired setting and gently tighten the thumbscrew. Tightening the thumbscrew is
optional; the aperture setting does not drift but can be accidentally changed if it is touched.
Fig 8. Aperture at f-stop 1.4 for UV light mode. Fig. 9. Aperture at f-stop 16 for white light mode.
2.7 Cable Setup
• Connect the PC cable. Connect the 9-pin connector to the Com 1 serial port of the
computer and the 25 pin connector to the system communications interface located in
the back panel of the Spot Cutter.
• Connect the AC power cord to the Spot Cutter instrument.
• Connect the camera power and communications cable to the PCI interface port on the
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