Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 42)

Note: Moving a Cut Circle
To move a cut request circle, make sure that Add Cut is selected, then position your cursor inside
the cut request circle. The appearance of the cursor will change to a 4-way arrow. Drag the circle
to a new location and release the mouse button.
• Microtiter plate well number
When the first spot selection is made, a dialogue box appears that asks for the starting
well number. The default is A1, but allows any starting well number to be entered. This
function allows a single microtiter plate to be more efficiently filled in order to optimize
downstream digestion robot use.
• Adding multiple cuts
This function allows the user to harvest a large amount of protein from a single spot and
deposit it in a single well. When the multiple cut box is activated, the add cut tool is used
to click and drag a box which defines a number of cuts within the box. Click and drag the
cursor over the spot to be cut. The software calculates how many cuts can be made in
the boxed area and marks each cut with a green circle. The user sets the maximum
number of multiple cuts to be made by entering a number in the "Max cuts/spot" box.
This limits the number of cut requests to the number indicated, regardless of how large
the box is drawn. All the cuts made with a click-drag are assigned to a single microtiter
plate well. Once the multiple cuts box is drawn, the user can move the cut location by
placing the cursor over the center cut request. The cursor icon will change to a 4-way
arrow. The user clicks and holds while moving the cut request to the desired location.
If multiple cuts from a single spot are desired in different microtiter plate wells, individually
select the cuts to default them to the next microtiter plate well. The multiple cut box is not
activated in this case, so that several individual cuts can be positioned within a single
spot. Each cut from the multiple cuts thus selected for a single spot are deposited into
separate microtiter plate wells.
• Removing a cut (or multiple cuts from the boxed area)
Activate the "Remove cut" icon, place the cursor over a selected cut and click. The
remaining cuts will not be re-numbered, but the next cut added is designated to the open
microtiter plate well. When removing a "multiple cuts" request, place the "remove cut" icon
directly over the center cut cut circle and click. When removing a number of spots at
once, click and drag a box around all the cuts to be removed at once with the "remove
cut" icon.
• Cuts Selected, Total Excisions, Total Plates
These headers provide feedback on the items listed.
Cuts selected denotes the number of cuts selected. Multiple cuts within a boxed area of
multiple cuts is counted as a single cut. In other words, cuts selected also refers to the
number of microtiter plate wells that will be filled.
Total excisions tracks the number of cuts made as the run progresses. This number
should equal the number of cuts selected when the run is completed.
Total plates counts the number of microtiter plates filled.
39