Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 18)

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The ProteomeWorks spot cutter (25 cm) model does not support the "Backed Gel" cutting
option. The software functions related to Backed Gels will be greyed out and unavailable for use.
Fig. 12. ProteomeWorks spot cutter Settings panel
If you select the ProteomeWorks Plus spot cutter model, set the “Crop left margin” to 50. Click
“Done” to save the changes and close the window.
The rest of the functions and set up are the same between the two models of spot cutter.
2.10 Camera Focus
Focus using the following steps.
• Loosen the 2mm hex screw in the silver lock collar of the lens with the red handled 2 mm
hex driver.
• Loosen the black aperture thumbscrew
• Set the aperture to the open setting of f-stop 1.4
• On the back portion of the spot cutter instrument, behind the cutting platform, is a black
toggle switch. This toggle switches the power to the white lights on or off. It is not the
mains power switch. Switch the toggle to the lights off position. Check that the lights are
off by pushing the green "Lights on" button on the lower front right side of the spot cutter.
If the platform lights come on, switch the toggle switch to the other position.