Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 41)

Fig. 37. Image Zoom and Transform.
Zoom and Transform
For any of the imaging options, the image tools can be used to enhance or zoom in on a region
of the image. On the right hand side of the screen are the zoom controls. The first icon on the top
is used to return the image to full view. The second icon down is used to magnify a region
specified by click-dragging a box around the region. The third icon down (the drag hand) grabs
the image to move to different locations within the image. The fourth icon down zooms in
centered on the placement of the icon, the fifth zooms out in the same way. The transform icon is
the last in the column of icons, and is used to adjust the brightness and contrast of the image.
5.3.2 Step 2: Specify cuts
This step activates the vertical arrow cursor tool for adding and removing cuts, allows for the
selection of multiple cuts, and has the snap to peak feature.
• Adding a single cut.
Using the zoom feature facilitates this process. First, zoom into the specified area, then
activate the Add Cut tool. To add a single cut move the vertical arrow cursor to the center
of the selected spot and click to designate a spot to be cut.
Once a spot is added, the add spot cursor tool changes to a 4-way arrow when placed
over a selected spot. Click and move the cursor to re-position the cut request. Each
selected cut request will be annotated with the microtiter plate well designated, and the x,
y coordinates of the cutting platform, if selected.
• Snap to peak
Snap to peak activates a pixel peak finding function within the software. This centers the
cut request on the darkest pixel area of the spot regardless where on the spot the cursor
is clicked.
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