Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 21)

2.11.2 Front/Back Centering of the Camera
• Replace the Calibration Template with the Camera Alignment Target template (clear plastic
sheet that contains one horizontal line). Remove the protective film from both sides of the
template.
• Click "Focus Cutter Camera" button if focus screen is not already on. You will see a single
horizontal line from the Template target in the camera image. The software displays a 5 x
5 grid pattern of red lines over this image on the screen. The next steps will show you
how to physically adjust the camera to center it over the cutting platform, using the
camera target line and the center (3rd), horizontal, red grid line.
• Loosen the set screw that slides the bracket of the camera mount and slide the camera
mount until the two center lines overlap. Hold the camera mount as it will sit when the
screw is tightened because it will affect the position of the camera.
• Tighten the set screw while maintaining the camera position.
• Click the "Stop" button to stop the focus mode and close the window.
Fig. 15a. Correct centering alignment.
2.11.3 Lateral Centering of the Camera
Acquire an image using the "Acquire Image" button. It may help to put an object (such as a hex
wrench) on the platform next to the right wall of the cutting area to make it easier to see the
border. Look at the right edge of the image. You should be able to see to just the edge of the
cutting area of the platform, but not into the microtiter plate area. If the camera image is too far to
the left or right of the edge of the platform, as in Fig. 15b, then adjust the side-to-side position of
the camera using the following procedure.
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