Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 8)

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Fig. 2. Cutting Platform and Cutting Area.
• ProteomeWorks Plus Cutting Mats, catalog number 165-7058.
One cutting mat is provided with the system. The cutting mats provide for a flexible
surface beneath the gel to allow for complete excision of the gel spots. The mats may
become scored by the cutting tip, stained by the gel stains, or become cracked with
repeated use over time. This is normal wear and replacement ProteomeWorks Plus
Cutting Mats should be ordered from Bio-Rad.
• ProteomeWorks Plus Gel Cutting Sheets, catalog number 165-7018. Gel Cutting Sheets
are thin plastic sheets that are positioned between the cutting mat and the gel in the
cutting area. This prevents scoring by the cutting tip and extends the life of the cutting
mat. The Gel Cutting Sheets can also be used to lift gels out of storage trays to minimize
damage to gels from excessive handling. (If a Gel Cutting Sheet will be used during gel
excision make sure that the cutting tip height is adjusted accordingly. See Section 7.4
"Set Cutting Tip Height".)
• Gel-holding bars
Two stainless steel Gel-holding Bars are included in the accessory box with the spot cutter.
These gel holding bars are used to immobilize the Coomassie stained PVDF membrane
during the X, Y calibration procedure, as well as for general gel excision. Correct placement
of the gel holding bars on the gel prevents gel movement during the gel excision process.
Position the bars resting on the outer edge of the gel or membrane. It is best to position
them to the left side and bottom of the gel so they are out of the cutting tip travel path.