Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 46)

Fig. 38. Confirm cuts window.
Answer "Yes" to bring up the following on-screen instructions for imaging the microtiter plate in
UV mode:
Fig. 39. Image Microtiter plate window.
2. Do not move the gel or membrane on the cutting platform
3. Place a clean plastic Gel Sheet on top of the gel
4. Place the microtiter plate on top of the plastic sheet. Note the orientation so that you can
recognize the A1 location.
5. Close the Enclosure door
6. Click on "Acquire image" when the plate is in place. The UV lights will turn on and the
camera will acquire an image with the same exposure time as the first spot cutter image.
7. Assess the image on the screen for the wells that need to be recut.
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