Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 20)

• Click "Stop" on the "Focus cutter camera" window before closing the window.
• Keep the light setting at the same "lights off" position for the camera alignment steps.
Any time the camera focus is changed, the spot cutter must be re-calibrated. The aperture can
be changed without re-calibrating.
2.11 Camera Alignment
(Tools needed from the accessory box: the 6 mm and the 3/16 inch mm hex driver)
2.11.1 Camera Rotation Alignment
• Place the Calibration Template (grid patter on clear plastic) on the cutting mat in the
cutting platform. Remove the protective film coating from both sides of the template.
• Click on the Focus Cutter Camera icon in the Basic Excision Tool screen. If the image is
over exposed, check the light and aperture settings. If the settings are correct, take the
cutting mat off the platform, click on "Auto-expose" and then click "OK" when "Auto-
expose completed". Place the cutting mat and Calibration Template back on the cutting
platform and proceed.
• Loosen the 6 mm camera mount screw slightly to be able to rotate the camera. The
screw is located on the under side of the curved arm supporting the camera.
• Rotate the camera mount until the black and red lines are parallel. Note, the lines will not
overlay exactly. See Figure 14 for an example
• Tighten the bottom mounting screw while holding the camera in the correct position. The
lines must remain parallel.
Fig. 14. Correct rotation alignment.
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