Bio-Rad PDQuest 2-D Analysis Software User Manual

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Fluorescent Enclosure

The cabinet

The door

Quick release hinges

Safety interlock switch

Base mat

UV-B lights

620 / 60 nm band pass filter

These parts are described in Figure 1.

Fig. 3. Fluorescent Enclosure with spot cutter.

Software Description

The ProteomeWorks Plus Spot Cutter requires PDQuest version 7.1.1 or greater for operation.
The ProteomeWorks Plus Spot Cutter is shipped with a Discovery Series install CD which
includes PDQuest software but does not include a License for PDQuest software. PDQuest
software runs the spot cutter in two modes.

The Basic Excision Tool provides the capability for imaging, calibrating, visually selecting
spots to cut, cutting them, and tracking the microtiter plate well they were deposited in. It
does not include any image analysis capability.

With a separate purchase of a license for full use of PDQuest image analysis software,
additional functionality is available. In addition to the use of the outstanding 2-D image
analysis features of PDQuest software, the full license provides the ability to use PDQuest
analysis sets to run spot excision, and all spot number tracking is kept within the PDQuest
matchset database. The manual spot selection of the Basic Excision tool is still available
as a menu option in the licensed version.

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