Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 25)

• To calibrate the microtiter plate position, place the cursor in the box value showing the X
or Y under "Well A1 position" and press the Enter key on the keyboard (the A1 position is
in the upper right corner of the holder). This will move the cutting tip to the A1 position of
the microtiter plate without lowering the cutting tip into the well, to prevent potential
damage to the cutting tip in cases of severe misalignment. DO NOT use Test button until
tip is centered using the following steps.
• Visibly assess where the cutting tip is in relation to the A1 well. The number values in the
boxes represent the x, y coordinates on the cutting platform (1 unit = 1 mm). Change the
x, y coordinates to position the cutting tip to the center of well A1. When entering a new
value, press return to move the cutting tip to the adjusted coordinates. To move the cutting
tip towards the front of the platform, decrease the Y value; to move towards the back of
the platform, increase the Y value. In order to move the cutting tip to the right, increase
the X value; to move to the left, decrease the X value. See Figure 19 below for a
description of the calibration movements.
• Click the "Test" button to assess the clearance into the microtiter plate well. The tip will
move to well A1 and H12. Once the tip is set to the A1 well, the interwell dimensions are
already set in the software, so the tip is calibrated for the entire plate. Press the "Done"
button to save the calibration changes.
Fig. 18. Calibration of Microtiter Plates.
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