Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 40)

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Note: Filter Install at Calibration
The software records the filter set-up with a confirmation button in the calibration. If the "Filter is
not Installed" button is clicked, because the filter has not been installed, then it indicates to the
software that the fluorescent enclosure is not being used. In this case, the UV light option in the
software will be grayed out and is not available. To activate the UV lights, the filter should be
added to the lens and the "Filter is installed" message is clicked at the beginning of the
calibration. This provides a record of how the installation was performed and the hardware that
was in place during calibration.
Note: UV lights Indicator Light
When the UV lights are active and the door is closed, the UV lights are confirmed to be active
with the external amber light on the side of the Enclosure nearest the door handle, labeled "UV
lights in operation". This light will turn on when the lights are actually in use during image
acquisition. The light will otherwise be off indicating that it is safe to open the door. A safety
interlock switch immediately stops power to the UV lights if the door is opened.
Note: Fluorescent Target
Once the Enclosure is installed, and the light settings and aperture are ready for imaging, a test
image may be desirable with a known target. Included in the Enclosure is a Fluorescent imaging
target. It is the square, clear plastic target with lines, squares and numbers on it. When imaged
with the UV lights, it should look like this on the computer screen:
Fig. 36. UV light exposure image of fluorescent target.
This target is also useful for focussing the lens during installation, re-calibration, or for checking
the image acquisition in UV or white light modes.