Bio-Rad PDQuest 2-D Analysis Software - Manual (Page 4)

Section 1
Introduction
1.1 General Description
The ProteomeWorks Plus Spot Cutter System is a precision instrument for the accurate
location, excision and loading into microtiter plates (MTP) of protein spots after separation on
2-D electrophoresis gels or PVDF membranes. The system has been designed to be both robust
in construction and simple to operate.
The ProteomeWorks Spot Cutter with Fluorescent Enclosure is capable of accurately cutting
spots from gels and membrane blots with visible stains such as silver and coomassie, and with
fluorescent SYPRO Ruby protein stain. The system includes a 620/60 nm bandpass filter that is
optimized for fluorescent SYPRO Ruby protein stain.
1.2 Manual Guidelines
If you are installing a ProteomeWorks Plus spot cutter and the ProteomeWorks fluorescent
enclosure together, use this instruction manual for the installation and operation of both devices.
If you are adding a ProteomeWorks fluorescent enclosure to an existing ProteomeWorks spot
cutter or ProteomeWorks Plus spot cutter, then use the Fluorescent Enclosure instruction manual
(part number 4006206) supplied with the enclosure.
1.3 System Components
The ProteomeWorks Plus Spot Cutter System is shipped in two boxes under the catalog order
number 165-7064. The spot cutter instrument and accessories box is catalog
number 165-7063 and the Camera System box is catalog number 165-7070.
The ProteomeWorks Plus Spot Cutter with Fluorescent Enclosure System is shipped in three
boxes under the catalog order number 165-7065. The spot cutter instrument and accessories
box is catalog number 165-7063, the fluorescent enclosure is catalog number 165-7044, and the
Camera System box is catalog number 165-7070.
In addition to the instrument boxes, 1 pack of precut PVDF membrane (162-0255) and
1 L of coomassie stain solution (161-0436) is shipped as part of this order and is used for
calibration of the spot cutter.
A focusing / fluorescent imaging target is included for use with the Fluorescent Enclosure and the
spot cutter. This target provides focussing lines for setting up the spot cutter camera. It also
provides fluorescent squares that can be imaged in the UV light mode. It is suggested that this
target be used to test the camera and UV lights before imaging a gel.
1