Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit - Manual (Page 13)

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b. Add 1.0 ml of prepared buffer A to the vial.
c. Replace the rubber stopper and gently invert the vial to solubilize the protein
standards.
2.4 Install the UNO Q1 column
Remove the end caps from the UNO Q1 column. Keeping tubing lengths to a
minimum, connect 1/16" tubing from port 4 of the AVR7-3 inject valve to the
column inlet. Connect the column outlet to the bottom of the UV flow cell or to
the QuadTec flow cell. Secure the column in a vertical position.
2.5 Prime the Pumps and Equilibrate the UNO Q1 Column
Ensure the pumps are stopped and the inject valve is in the purge position.
Re-prime and purge pumps A and B as described in Section 1.1 of this manual.
Set the inject valve to position L (Load). Set the flow rate to 2.0 ml/min. Set the
UV range to 0.1 AUFS and the conductivity range to 100 mS/cm.
a. Wash the column with 6.5 ml (5 column volumes) of buffer B at 2 ml/min.
b. Equilibrate the column with 13 ml (10 column volumes) of 100% buffer A.
The conductivity monitor on the status bar should now read
≤ 3 mS/cm.
2.6 Create a New Method
In the Manual screen, select the Browser icon from the tool bar. In the Browser
screen you will enter a user name for your method (refer to page 6-1 of the
DuoFlow instruction manual for more information on the Browser screen)
according to the following steps:
• Select the Browser icon from the tool bar menu
• Select the New icon from the upper left side of the Browser screen
• Select New from the drop-down menu and enter your user name in the dialog
box.
• Click on the Project icon for your user name
• Select New and New Method. Enter your method name (or use default
Method 1)
• Click OK to proceed to the instrument/devices Setup screen
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