Bio-Rad BioLogic DuoFlow Pathfinder 80 System DuoFlow Chromatography System Starter Kit User Manual
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3.1 Prime the Workstation Pumps
a. Immerse Maximizer inlet lines A1, A2, B1, and B2 in a container of HPLC
grade (filtered, degassed) or other high-quality water.
b. From the Manual screen place the Maximizer in Local mode and use the
Valve Port Select button, under the A1/A2 Maximizer valve inlet, to select
inlet port A1.
c. Connect the syringe (supplied with the fittings kit) to the priming port of
pump A.
d. Turn the priming port counter-clockwise one full turn to open the seal. Gently
withdraw the syringe plunger to draw water into the pump head.
e. Repeat this operation several times until no air bubbles are visible in the inlet
tubing.
f.
Use the Valve Port Select button on the Maximizer to select solution A2.
Gently withdraw the syringe plunger to draw water into the pump head.
g. Repeat this operation several times until no air bubbles are visible in the inlet
tubing.
h.
Tighten the priming port by turning it clockwise.
i. Repeat this priming procedure for pump B and inlets B1 and B2.
3.2 Move the AVR7-3 Inject Valve to the Purge Position
Prior to purging the pumps at 10 ml/min it is essential to place the AVR7-3 valve
in the purge position. This directs the flow to waste and not to the columns and
detector.
To change the position of the AVR7-3 inject valve, select P from the Manual
screen valve control panel for the AVR7-3 valve. Select the workstation or
Maximizer valve control panel that displays the AVR7-3 inject valve. For example, if
you have connected the AVR7-3 inject valve into port 10, you will see a valve box
designated AVR7-3 at port 10 of the Maximizer valve control panel. The three
buttons of this box correspond to valve positions as follows: L = Load position,
I = Inject position, P = Purge position. To move the AVR7-3 valve to Purge
position, click button P.
The default position at power up and at the end of a programmed method for the
AVR7-3 is L. For all other automated valves the default is position 1.
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